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| cell fractionation ： ウィキペディア英語版 |
cell fractionation
Cell fractionation is the separation of homogeneous sets from a larger population of cells.
==Homogenization==
Tissue is typically homogenized in an isotonic buffer solution, as well as a pH buffer by use of a variety of mechanisms such as grinding, mincing, chopping, pressure changes, osmotic shock, freeze-thawing, and ultra-sound homogenization. This is done to stop osmotic damage. The samples are then kept cold to prevent enzymatic damage .
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